GeneArt® Gene Synthesis – Order form, enter and optimize a sequence (tutorial)

This tutorial describes how to order a gene
synthesis including sequence editing and GeneOptimizer online optimization for maximum expression
yields. Begin with the “Enter sequence” tab. Enter the name, select the sequence type,
enter the sequence and select the bio safety level. For further details see the tutorial: “overview
of funtions” Click the “Edit sequence” tab to define
your open reading frame. The ORF function allows you to either select
from a pre-set of detected ORFs or to customize your ORF. Please see the “Show more” feature for
further ORFs. You can also add stop codons to your sequence
or delete them. Your selected ORF will be highlighted in the
sequence and also added to the feature map. The next step is to select your cloning sites. You can either select from a pre-set of detected
sites or add new sites. Please select the 5 prime cloning site you
want to use. Selected sites will remain unchanged during
optimization and the motif will not get generated in the optimized sequence. Select your 3 prime cloning site accordingly. Go to the “Optimize sequence” tab and
define which expression host the sequence shall be optimized for. Please note that depending on the expression
organism, a feature will be available for adding the appropriate Kozak sequence. To receive a specialist optimization, click
here. Ensure all sequence editing and specification
is complete. Then click the “Optimize” button to begin
online optimization. The duration of optimization will depend on
the sequence length and complexity. The sequence summary tab will show your sequence
optimized for the requested organism. The sequence can be downloaded in various
formats. The summary can be downloaded as PDF. Graphical data displays the quality of your
sequence before and after optimization. Please note, you can return to the previous
tabs to make modifications at any time. Remember to click the optimize button each
time. Once completed click “Save & Close”. You are now ready to add your project to the
cart. View the “Add to cart” tutorial to see
how that process works.

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