Lac Operon – gene regulation in prokaryotes
The primary control framework for protein
generation worked out at the molecular level depicted the control of chemicals that are
delivered in light of the nearness of the sugar lactose in E. coli cell. The work was
performed by Jacob and Monod for which they were honored the Nobel Prize. The accompanying
is the pathway that prompts the generation of glucose and galactose. A few proteins required in lactose digestion
system in the E. coli cell. They are: � �-galactosidase – changes over lactose
into glucose and galactose � �-galactoside permease – transports
lactose into the cell � �-galactoside transacetylase – capacity
obscure Research with this framework was incredibly
included by the accessibility of constitutive mutants. A constitutive mutant is one in which
the quality item is created persistently, that is there is no power over its look. In
these mutants, the above proteins were created all the time in contrast with the wild sort
where the proteins just showed up within the sight of lactose. So in these mutants, the
change must be a quality other than those in charge of the auxiliary qualities. The greater part of the qualities required
in controlling this pathway are situated alongside each other on the E. coli chromosome. Together
they frame an operon. The accompanying is the hereditary structure of the operon. Operon – a bunch of auxiliary qualities that
are communicated as a gathering and their related promoter and administrator How does the framework work? Without lactose
in the cell, the repressor protein ties to the administrator and keeps the read through
of RNA polymerase into the three auxiliary qualities. With lactose in the cell, lactose
ties to the repressor. This causes an auxiliary change in the repressor and it loses its liking
for the administrator. Along these lines RNA polymerase can then tie to the promoter and
translate the auxiliary qualities. In this framework lactose goes about as an effector
particle. Effector atom – a particle that associates
with the repressor and influences the partiality of the repressor for the administrator With the above data, we can now anticipate
the impact that different mutants will have on lac operon quality expression. Catabolite Repression of the lac Operon Lactose is not the favored starch hotspot
for E. coli. On the off chance that lactose and glucose are available, the cell will utilize
the greater part of the glucose before the lac operon is turned on. This sort of control
is termed catabolite repression. To avert lactose digestion system, a second level of control of quality expression
exists. The promoter of the lacoperon has two restricting destinations. One site is
the area where RNA polymerase ties. The second area is
the coupling site for a complex between the
catabolite activator protein (CAP) andcyclic AMP (cAMP). The official of the CAP-cAMP complex
to the promoter site is required for translation of the lac operon. The nearness of this complex
is nearly connected with the nearness of glucose in the cell. As the centralization of glucose
expands the measure of cAMP abatements. As the cAMP abatements, the measure of complex
reductions. This diminishing in the complex inactivates the promoter, and
the lac operon is killed. Since the CAP-cAMP
complex is required for interpretation, the complex applies a positive control over the
statement of the lac operon.
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U r magical. I love u
Excellent sir
Thanks sir
this is from my heart…thankyou so much… i was struggling a lot with these concepts…..
Nice explanation sir
??????
sorry to say but you made it more complicated
Lac operon shows negative regulation
sir mujhe ye confiussion hai k where lac operon is situated and function of inducer plz help me
Well explained….
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valobasa
Best
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ur power point work also good ( in Hindi)..
Wow, i was able to perfectly understand this process with your help. Thanks
Sir i have a doubt uh said that if lac operon is on firstly allulactose binds to repressor and change its shape and then so on but how this lactose bind the repressor because with the help of permiase lactose enter but before it permiase is not synthesized so how it will enter and bind to repressor
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सर थोड़ा हिन्दी मे भी बताने की कृपा करे
I am speechless . The way of is ossem
I like your smile while teaching ?
Hindi me video bnaiye na pls sir???
it very easy method– thanks
Kya falthu pada raha hai main baat too bataya hii nahi….
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I am a PhD Biotechnology student.I am at least following one video of u for clear understanding of some lectures.All you vidieos are excellent .Keep it up
Thanks u so much sr for this video
i am also a die heart fan of you….you always save me before my examinations…the way you teach or elaborate is really easily understandable…and in the examination i always forget what i have studied but only remember you words and your examples that you used in your videos..so thanks a lot
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Good explanation
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Always you speak in English bro that helps me lot
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Sir ??
Thank you so much sir….. Its very helpful to all of us
Currently a Biomedicine student, your videos are incredible and are alot easier to understand the information from. Your videos have helped with my assignments and exam revision. You sir are much better than my PhD lecturers at university here in the UK.
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Quality content. I have no idea why i'm learning this, though.
Sir plz upload a video for ,Hormones as a mediator for development
Sir,I'm from Pune university doing Bsc in Zoology….Your explanation is too gud sir plz come to our university…..Your videos helped m alot???..
Amazing explanation. You have a gift and passion for teaching. Thank you!
Thnkuu soo mch sir….m a biotechnology student …. Earlier lac operon was a huge hurdle for me…i was unable to understand…….. Bt aftr watching ur video… Uh made me understood soo easily… Nw fr me lac operon is soo easy like anything….. Thnxx a lot….
Thank You. The way you explain Biology shows me that you have a deep understanding. Because knowing is much different from understanding. Any tips that can help me understand Biology concepts rather than just know ?
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Thank you sir
I watched this video when I was studying 12th std and I’m watching it now when I’m studying mbbs 1st year..you’re always the best!!!
Very clearly explained thank you
Great video! thank you for explaining this.
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Hmm
to be honest yr video was lit , all points are brilliantly explained cleared all my doubts thnx a lot
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Tbh I am really a huge fan of your teaching and so I tried to contact you for coaching also ..but the way you seenzoned and avoided me ? after that even if I see your videos , I can't respect and watch ..I can't judge you but I guess everyone should be more down to earth to reply to their students 🙂 thank you
Salute their efforts…
Thank you. sir
Best explanation Sir….God Bless you!!!
Thank you so much for these lectures.Sir what are the important topic releated to jnu 2019 entrance.Please give me idea.
Thank u Sir
Excellent ?
Thanku bhai
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Very simply explained..
Intersting way of teaching.
Keep posting ….
Sooo nice…tq
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Ur teaching is best, kindly made all chapters of biology in live (not in ppt form) , hope for earliest
Thank you?
Sir repeatedly u use to say transcribe the protein … But sir in place of trascription it is translation of protein…
But sir ur videoes are much helpful in throughout my bsc carrier… Thank u so mch sir
Sir can u please make a video on nucleic acid conversion factor
Sir are you have any coaching centre in Garia Kolkata??
Inhibitor binds with promotor or operator?
Such an awesome explanation.. Thank you sir..!!
Thank you sir
Awesome Sir:-)
So easily explained .thankyou sir for this video .
Thank you bhaiya.. Understood well.. But one question… How much quantity of these 3 protein produced?? Plz answer
I'm currently in class 12 and finding much difficulty to understand this concept in Molecular basis of Inheritance chapter…And thus I discover a great channel for clearing my bio doubts..Thanx a lot sir?
It is just aaosum
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Excellent, it was good
Transacetylase : an enzyme coded by Lac A .. just as you said.
Although its physiological role is still not completely clear, it may be involved in the removal of toxic by-products of lactose digestion from the bacterial cell. Credit: Essential of genetics 9th edition, Page 282
thanks for the video, it is very helpful.
Hi sir, I am from Burdwan Medical College and Hospital in India… it is very helpful to me to study Biochem subject.. Excellent!! Thank you Sir
i can literally watch the video in 1.75x speed and still understand it..
Greetings for Germany. : )
informative
Thank you sir……
Thank you very much?
My best biology's teacher ever..?, btw thank you so much ?
Lots of Love from MBBS students❤️❤️❤️
Thanks so much from a médecine student from belgium!!!
Bhaiya thankuuuuuuu u….
sir..the repressor protein that is produce from laci ,placed at operator or promoter site?? this concept is not clear to me..?
Very good
I am a 12th class kid preparing for NEET exam… I found this video very useful.. Thanks a lot sir.. I have seen ur other videos too.. U r doing excellent.. Keep going sir
Thank you ? ur videos are awesome ?
awesome explanation…very easy to understand tq sooo much bro..
The product of lac i gene bind to operatar then u say it bind both promoter and operator